Osmotic Fragility and Viability of Lysostaphin-induced Staphylococcal Spheroplasts
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چکیده
منابع مشابه
III RESULTS OF TESTS FOR HAEMOLYSIS AND FOR OSMOTIC FRAGILITY OF RED BLOOD CELLS Osmotic Fragility
SYNOPSIS Nineteen patients with morphological abnormalities of the red blood cells are described, and these formed approximately 3 % of the total cases of cardiac valvular disease. In two patients the abnormal blood film developed after the insertion of an aortic and mitral valve prosthesis respectively, but in another two patients the abnormal blood film was corrected by aortic valve surgery. ...
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Serious staphylococcal infections continue to pose therapeutic problems despite the availability of antimicrobial agents that are effective against staphylococci in vitro. Much suggests that this relates, in part, to the nature of the basic lesion in staphylococcal disease. The abscess is the hallmark of staphylococcal disease, and many therapeutic failures appear to result from the inability o...
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Fractionation of highly purified staphylococcal delta hemolysin by sucrose density gradient centrifugation, Sephadex G-150 and Bio-Gel A-5m gel filtration, carboxymethyl cellulose chromatography, and isoelectric focusing failed to separate the hemolytic, protoplast-lysing, and spheroplast-lysing activities of the preparation. The activities were reduced to a similar extent when delta hemolysin ...
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A precise and highly reproducible method for analyzing the osmotic fragility of erythrocytes with a minute amount of blood (less than 10 mul) is described. The osmotic fragility curves are recorded with a coil planet centrifuge with accessories and a scanning photodensitometer. The recorded curves are transcribed by a DuPont Curve Resolver and their components are analyzed. Normal fragility cur...
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Physiological states associated with inositol starvation of spheroplasts of Saccharomyces cerevisiae were investigated and compared with conditions preceding death of starved whole cells. In the absence of synthesis of inositol-containing lipids, cell surface expansion terminated after one doubling of whole cells. In spheroplasts, cessation of membrane expansion was apparently followed by rapid...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1968
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.96.3.734-737.1968